Monday, January 23, 2012

Fluorometry Molecular Fluorescence


Fluorometry:  Molecular Fluorescence 
Principle :  This technique utilises the phenomenon  of molecular fluorescence, the theory 
behind this is exactly the same that has been discussed under atomic fluorescence but through 
the excitation of bonded electrons. Here, most often the irradiating light is in the range of 
ultraviolet and visible.  

Instrumentation :  The instrumental set-up comprises of a UV/Visible source, two 
monochromators, detector and recorder. The fluorescence exhibited by the sample is 
measured at right angles to the incident beam. The following is the basic set-up: 



Applications :  The applications of this technique are limited and it offers quantitative 
estimations of those compounds like benzene and fused benzene ring systems. Inorganic 
metals can also be analysed by the ability of  them to form complexes with the ligands. It 
finds uses in the analysis of foods for vitamin content, since vitamins like riboflavin, niacin, 
etc., exhibit fluorescence.

Only limited  compounds show the fluorescence hence this technique is relatively free of any interference and is very sensitive.

Disadvantages : The application is very limited as relatively a few substances exhibit
flourescence.



No comments:

Post a Comment