High-Performance Partition Chromatography: It is the most widely used liquid
chromatographic procedures to separate most kinds of organic molecules. Here the
components present in the analyte mixture distribute (or partition) themselves between the
mobile phase and stationary phase as the mobile phase moves through the column. The
stationary phase actually consists of a thin liquid film either adsorbed or chemically bonded
to the surface of finely divided solid particles. Of these the latter is considered more
important and has a distinct stability advantage. It is not removed from the solid phase either
by reaction or by heat and hence it is more popular. It finds wide applications in various
fields, viz., pharmaceuticals, bio-chemicals, food products, industrial chemicals, pollutants,
forensic chemistry, clinical medicine, etc.
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