High-Performance Adsorption Chromatography: Here the analyte species (components
to be analysed) are adsorbed onto the surface of a polar packing. The stationary phase
consists of finely divided solid particles packed inside a steel tube. If the component mixture
is eluted through this tube with the mobile phase, different components present in the mixture
adsorb to different degrees of strength and they become separated as the mobile phase moves
steadily through the column. The nature of the adsorption involves the interaction of polar
molecules with a very polar solid stationary phase. The stationary phase could be silica gel or
alumina. This method is extensively used for the separations of relatively non-polar, water-
insoluble organic compounds (since polar molecules will be adsorbed on to the column
momentarily).
One particular application is in resolving isomeric mixtures such as meta- and
para-substituted benzene derivatives.
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