ANALYSIS THROUGH CHROMATOGRAPHY
The technique through which the chemical components present in complex mixtures are
separated, identified and determined is termed as chromatography. This technique is widely
used like spectroscopy and is a very powerful tool not only for analytical methods but also
for preparative methods. Compounds of high grade purity can be obtained by this method.
Chromatography can be simply defined as follows:
“It is the technique in which the components of a mixture are separated based upon the
rates at which they are carried or moved through a stationary phase (column) by a gaseous
or liquid mobile phase”.
Based on the mobile phase this technique can be simply classified into two categories as:
Liquid Chromatography and Gas Chromatography. The column which holds the stationary
phase (which in the form of small particles of the diameter of the order in microns), plays
unique role in these processes. Usually silica is the base material for producing this phase.
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