Fluorometry: Molecular Fluorescence
Principle : This technique utilises the phenomenon of molecular fluorescence, the theory
behind this is exactly the same that has been discussed under atomic fluorescence but through
the excitation of bonded electrons. Here, most often the irradiating light is in the range of
ultraviolet and visible.
Instrumentation : The instrumental set-up comprises of a UV/Visible source, two
monochromators, detector and recorder. The fluorescence exhibited by the sample is
measured at right angles to the incident beam. The following is the basic set-up:
Applications : The applications of this technique are limited and it offers quantitative
estimations of those compounds like benzene and fused benzene ring systems. Inorganic
metals can also be analysed by the ability of them to form complexes with the ligands. It
finds uses in the analysis of foods for vitamin content, since vitamins like riboflavin, niacin,
etc., exhibit fluorescence.
Only limited compounds show the fluorescence hence this technique is relatively free of any interference and is very sensitive.
flourescence.
No comments:
Post a Comment