Monday, January 23, 2012

Atomic Fluorescence Fluorometry

Fluorometry : Atomic Fluorescence 
This technique is not widely used though its counterpart -  the molecular fluorescence is 
applied well to the analytical studies.



 The principle of atomic fluorescence is that when atoms are elevated to higher energy levels, they  sometimes return to the ground state through a 
pathway, which has several intermediate electronic states, before reaching to the actual 
ground state.
 Such series of fall through the electronic levels accompany by light emission - 
which is atomic fluorescence.
 The intensity of this emitted light is measured at right angles to 
the incident light and related to concentration.
Uses are similar to AAS and AES. 

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